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. 2013 Oct 29;7(1):101–106. doi: 10.3892/ol.2013.1645

Figure 3.

Figure 3

IL-6 expression in AEG-1 knockdown cells, and proliferation and apoptosis in HepG2-shAEG-1 cells treated with IL-6. (A and B) IL-6 expression was detected by qPCR and ELISA in the HepG2-shAEG-1 and HepG2-vector cells (*P<0.05). (C and D) Apoptosis analysis of the HepG2-shAEG-1 cells that were treated with IL-6 (50 ng/l) was assayed by flow cytometry (*P<0.05). (E) The proliferation of the HepG2-shAEG-1 cells that were treated with IL-6 (50 ng/l) or remained untreated was detected by MTT assay (*P<0.05). Error bars represent SD, n = 3 experiments. AEG-1, astrocyte-elevated gene-1; qPCR, quantitative polymerase chain reaction; ELISA, enzyme-linked immunosorbent assay; MTT, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide.