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. 2013 Nov 18;7(1):131–136. doi: 10.3892/ol.2013.1685

Figure 1.

Figure 1

Identification of EGFP-labeled lentiviral expression vector. (A-1) Gel electrophoresis of the PCR product of pLenti6.3-EGFP-EGFR-miR. M, marker DL2000; lane 1, negative control; lanes 2–4, PCR product of recombinant vector (911 bp). (A-2) Restriction map of the PCR positive vector of pLenti6.3-EGFP-EGFR-miR. M, marker DL15000; lane 1, digestion product of double restriction endonuclease of AscI and PmeI; lane 2, the recombinant plasmid pLenti6.3-EGFR-EGFR-miR. (B-1) Gel electrophoresis of the PCR product of pLenti6.3-EGFP-FGFR-miR. M, marker DL5000; lane 1, negative control; lanes 2–6, PCR product of recombinant vector (911 bp). (B-2) Restriction map of the PCR positive vector of pLenti6.3-EGFP-FGFR-miR. M, marker DL5000; lane 1, digestion product of double restriction endonuclease of AscI and PmeI; lane 2, the recombinant plasmid pLenti6.3-EGFP-FGFR-miR. EGFP, enhanced green fluorescent protein; EGFR, epithelial growth factor receptor; miR, microRNA; PCR, polymerase chain reaction; FGFR, fibroblast growth factor receptor.