Figure 3.

Expression of EGFR and bFGFR in U251 cells evaluated by quantitative polymerase chain reaction (qPCR). A decrease in EGFR mRNA expression was observed in U251 cells after transfection with Lenti-EGFP-EGFR-miR and Lenti-GFP-EGFR-miR + Lenti-EGFP-FGFR-miR [comparison with cell control and RNAi-negative control (NC); P<0.005]. The downregulated mRNA expression of bFGFR was also detected in U251 cells transfected with Lenti-EGFP-FGFR-miR and Lenti-EGFP-EGFR-miR + Lenti-EGFP-FGFR-miR (^*P<0.005; comparison with cell control and RNAi-NC). EGFP, enhanced green fluorescent protein; EGFR, epithelial growth factor receptor; miR, microRNA; bFGFR, basic fibroblast growth factor receptor.