FIGURE 2.

Chimeric mutation of TRPV1 and TRPV3. A, representative heat-evoked current traces from HEK293 cells expressing mouse TRPV3. B, schematics of chimeras of TRPV1 and TRPV3. In TRPV1 ntV3, TRPV1 Met¹–Val⁴³⁰ were replaced by TRPV3 Met¹–Ala⁴³⁷. In TRPV1 pV3, TRPV1 Asp⁶⁰¹–Val⁶⁵⁸ were replaced by TRPV3 Lys⁶¹¹–Leu⁶⁵³. In TRPV1 ctV3, TRPV1 Asn⁶⁸⁷–Lys⁸³⁸ were replaced by TRPV3 Glu⁶⁸²–Val⁷⁹¹. C, Western blot analysis of biotinylated or soluble proteins in HEK293 cells expressing cDNAs as indicated. FLAG-tagged TRPV1 was detected by specific antibody against FLAG. GAPDH was used as a loading control. D and E, relative responses from TRPV1 and chimeras. The peak current amplitudes were normalized to the average of TRPV1 responses by heat (45 °C, 30 s; D) or capsaicin (10 μm; E). The recording solutions were int A/ext A. *, p < 0.05; **, p < 0.005; ***, p < 10⁻³ in Student's t test. The numbers in parentheses represent the numbers of observations.