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Sedimentation equilibrium analysis of a PIV5 F TM domain peptide confirms a monomer-trimer equilibrium. The PIV5 TM peptide was prepared in C14SB detergent (protein:lipid molar ratio 1:280), and A280 data were collected at 40,000 rpm on a Beckman XL-1 analytical ultracentrifuge. A and B, the predicted curves (dark lines) for monomer (A) and monomer-trimer (B) fits are shown and superimposed with actual data points.
