FIGURE 7.

Mutations at Hendra F Gly-508 can modulate expression of the cleaved, fusogenic form. A, cell surface expression of transiently transfected wild type and Hendra F mutants in Vero cells following a 3-h metabolic label. Surface proteins were biotinylated prior to immunoprecipitation, and the total and surface populations were separated by streptavidin pulldown. Proteins were analyzed via 15% SDS-PAGE and visualized using autoradiography. B, cell surface expression (CSE) and fusion for the wild type and Gly-508 mutants. F₁ band quantitation via densitometry was normalized to wild-type levels plus or minus one standard deviation. Cell surface expression represents the average of three independent experiments. Fusion was analyzed using a reporter gene assay, n = 9; ± S.E.