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. 2013 Oct 31;288(50):35726–35735. doi: 10.1074/jbc.M113.514554

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Mutations at Hendra F Ser-493 inhibit fusion. A, cell surface expression of transiently transfected wild type and Hendra F mutants in Vero cells following a 3-h metabolic label. 2A = T511A/S514A; 3A = S501A/T511A/S514A; and 4A = S493A/S501A/T511A/S514A. Proteins were analyzed via 15% SDS-PAGE and visualized using autoradiography. B, cell surface expression (CSE) and fusion for the wild type and TM mutants. F₁ band quantitation via densitometry was normalized to wild-type levels plus or minus one standard deviation. Cell surface expression represents the average of three independent experiments. Fusion was analyzed using a reporter gene assay, n = 5–9; ± S.E.