TABLE 6.
Sensitivities of wild-type and mutant GS proteins to inhibition
The IC50 is the inhibitor concentration that reduces enzymatic activity by 50%. The Mg2+-dependent biosynthetic reaction was used to measure the inhibition. All values are the averages of at least two determinations. The S.E. values were less than 10% for all values. The values for the wild-type and E304A enzymes are taken from Ref. 40.
| Enzyme | IC50 |
||
|---|---|---|---|
| Glutamine | AMP | Met-Sox | |
| mm | |||
| Wild type | 2.4 | 0.5 | 0.13 |
| R62A | >140 | 20 | 0.19 |
| E304A | >140 | >30 | 26 |