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Electrostatic potential and field lines calculated using the adaptive Poisson-Boltzmann solver software (24) for wild-type ASIC protein (A); pair 260/354 mutated to alanines (B); pairs 238/350 and 239/346 mutated to alanines (C); and pairs 238/350, 239/346, and 260/354 mutated to alanines (D).
