FIGURE 8.

EBF1 knockdown impairs insulin signaling in adipocytes. A, expression of selected insulin signaling genes in EBF1-deficient 3T3-L1 adipocytes by qPCR. Error bars, S.E.; *, p < 0.05. B, insulin signaling is impaired in EBF1-deficient cells. EBF1 knockdown or control cells were treated with or without 10 nm insulin for 30 min and then harvested with lysis buffer and subjected to SDS-PAGE/immunoblotting with the indicated antibodies. Each transduction was performed in triplicate. Experiments were performed with scrambled hairpin versus shEbf1-A. C, EBF1 knockdown impairs insulin-stimulated glucose uptake. Data are normalized to total protein; transductions were performed in quadruplicate. Error bars, S.E. Experiments were performed with scrambled hairpin versus shEbf1-A. D, EBF1 knockdown impairs insulin-stimulated lipogenesis. Data are normalized to total protein obtained in each sample, and each infection was performed in quadruplicate. Experiments were performed with scrambled hairpin versus shEbf1-A. Error bars, S.E.