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Schematic illustrations of the QCM experimental setup. A, a 27-MHz QCM device (AFFINIX Q4) for observation of the thiol-disulfide exchange reaction of reduced DsbASH,SH with biotin-BCCP-DsbBS-S,S-S embedded in a lipid bilayer on a NeutrAvidin-modified QCM plate. B, preparation of a supported lipid bilayer around biotin-BCCP-DsbBS-S,S-S immobilized on a NeutrAvidin-modified QCM plate. C, kinetic parameters (kf, kr, and kcat) measured in this work.
