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. 2013 Oct 21;288(50):35969–35981. doi: 10.1074/jbc.M113.519876

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Effects of the two cysteine residues (Cys-41 and Cys-44) of DsbB_S-S,S-S for the reaction with DsbA_SH,SH. A, typical time courses of frequency changes in the QCM on which DsbB(C41A,C44A)_S-S embedded in supported bilayers was immobilized in response to the addition of DsbA_SH,SH (a) and DsbA(C33A)_SH (b). The experimental condition was the same as that of reactions between DsbA_SH,SH and DsbB_S-S,S-S in Fig. 3A. B, saturation curves of mass increases due to the intermediate formation between DsbA(C33A)_SH and DsbB_S-S,S-S (a) or DsbB(C41A,C44A)_S-S (b) immobilized on a QCM. Maximum binding amounts (−ΔF_max) were calculated to be 321 ± 12 Hz for DsbB_S-S,S-S and 392 ± 78 Hz for DsbB(C41A,C44A)_S-S with experimental errors, respectively.