TABLE 1.
Strains
| Strain or plasmid | Genotype or descriptiona | Source |
|---|---|---|
| E. coli | ||
| DH5α | F−r−m+Ø80dlacZΔM15 | Invitrogen |
| BL21 (DE3) | Expression host, inducible T7 RNA polymerase | Novagen |
| S. agalactiae | ||
| COH31 | Serotype III | 67 |
| PS954 | COH31Δsrr1, CmR | 15 |
| NCTC 10/84 | Serotype V, clinical isolate | 68 |
| PS2645 | NCTC 10/84Δsrr1, CmR | 15 |
| COH1 | Serotype III, clinical isolate, ST-17 | 69 |
| PS2641 | COH1Δsrr2, CmR | 17, 49 |
| PS2931 | PS2641/pDE-Srr1 | This study |
| PS2933 | PS2641/pDE-Srr2 | This study |
| H36B | Serotype Ib | 70 |
| NCTC 1/82 | Serotype IV | 4 |
| J48 | Serotype III, ST-17 | 16 |
| NEM316 | Serotype III, ST-23 | 71 |
a ErmR, erythromycin resistance; CmR, chloramphenicol resistance.