FIGURE 1.

Inhibition of glycolysis, but not mitochondrial metabolism, induces cell death in pancreatic cancer cells. PANC1 cells were treated with either the mitochondrial inhibitor, CCCP (1–10 μm, A), the glycolytic inhibitor, BrPy (100–1000 μm, B), or a combination of both (A, filled diamond) for varying times (0.5–6 h). Cell death was determined using PI fluorescence on a Cellomics® ArrayScan® VTI HCS Reader (Imagen Biotech). PI-positive cells (dead) were normalized to Hoechst-positive cells (cell count) to determine % cell death (n = 5). **, p < 0.01; ***, p < 0.001 compared with control (two-way analysis of variance, post hoc Bonferroni correction).