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. 2013 Oct 28;288(50):36052–36060. doi: 10.1074/jbc.M113.522441

FIGURE 5.

FIGURE 5.

EPR spectra manifested by the silver-substituted CO dehydrogenase under various conditions. A, CO-reduced silver-substituted CO dehydrogenase in 50 mm HEPES, pH 7.2, with simulation in red. B, CO-reduced silver-substituted CO dehydrogenase in 50 mm HEPES, pD 7.2. C, dithionite-reduced silver-substituted CO dehydrogenase in 50 mm HEPES, pH 7.2. Brackets indicate approximate g1,2,3 values (dashed lines) with copper splittings (solid lines). The EPR instrument settings were: 9.45 GHz microwave frequency; 4 milliwatt microwave power; 5 Gauss modulation amplitude; 150 K.