TABLE 2.
Primers used in this study
Name | Sequence 5′ → 3′ |
---|---|
Primers used for construction of deletion mutants | |
Delare1fw | GTTCAGCACGGCTTGCAGCAAGAGCGCCAAAACAGATTGCAAGACAGCTGAAGCTTCGTACGC |
Delare1rev | TATATCTATCAAGGGCTTGCGAGGGACACACGTGGTATGGTGGCAGTGCATAGGCCACTAGTGGATCTG |
Delare2fw | TATATCTATCAAGGGCTTGCGAGGGACACACGTGGTATGGTGGCAGTGCATAGGCCACTAGTGGATCTG |
Delare2ev | AACAGACACATTACGTTAGCAAAAGCAACAATAACAAACACAACCCAGCTGAAGCTTCGTACGC |
Are1fwctrl | GAAAAATGTGAGATGGTGTAGAGTG |
Are1revctrl | ATGGTTCTGCCCCAGATTTACC |
Are2fwctrl | CTTTCATCAATACATCTATATATTCG |
Are2revctrl | GTAATTGTGGTAGCTGTGTGTTCAT |
Primers for construction of overexpressing constructs | |
Lpx1fw | GCGTGGATCCATGGAACAGAACAGGTTCAAGAAAG |
Lpx1rev | CGGGAATTCTTACAGTTTTTGTTTAGTCGTTTTAACC |
Ldh1fw | GCGTGGATCCATGAATATGGCAGAACGTGCAG |
Ldh1rev | CGGGAATTCCTACAATTTGGAATTATCAATCACCTCTCG |
Yju3fw | GCGTGGATCCATGGCTCCGTATCCATACAAAG |
Yju3rev | CGGGAATTCTTATGGTTTAGCTTCGGTCGTG |
Ayr1fw | GCGTGGATCCATGTCGGAGTTACAGTCACAAC |
Ayr1rev | CGGGAATTCCTAATCGTCCTTATTCTTCTGTTTCGAC |
Eht1fw | GCGTGGATCCATGTCAGAAGTTTCCAAATGGCC |
Eht1rev | CGAGTCGACTCATACGACTAATTCATCAAACTTAGTG |
Tsc10fw | GCGTGGATCCATGAAGTTTACGTTAGAAGACCAAGTTG |
Tsc10rev | CGAGTCGACTCAGTTGGCCTTCTTGCCGTC |
YBR056wfw | GCGTGGATCCATGATTGGCTCACTTAGAAACAAATTTGAG |
YBR056wrev | CGGGAATTCTTAATACTTATTAAACTCATCTAACCCACGTTG |
YKL050cfw | GCGTGGATCCATGTCACTAATATCTGCGTTGCAAAC |
YKL050crev | CGAGTCGACTTAGATAACCTCTTTGAAAAAACTTTCCTTAGGAG |
Primers for site-directed mutagenesis | |
Ayr1_S18Afw | GTTGTTACAGGCGCCGCCGGTGGTATTGGATATG |
Ayr1_S18Arev | CATATCCAATACCACCGGCGGCGGCTGTAACAACG |