TABLE 2.
Primers used in this study
| Name | Sequence 5′ → 3′ |
|---|---|
| Primers used for construction of deletion mutants | |
| Delare1fw | GTTCAGCACGGCTTGCAGCAAGAGCGCCAAAACAGATTGCAAGACAGCTGAAGCTTCGTACGC |
| Delare1rev | TATATCTATCAAGGGCTTGCGAGGGACACACGTGGTATGGTGGCAGTGCATAGGCCACTAGTGGATCTG |
| Delare2fw | TATATCTATCAAGGGCTTGCGAGGGACACACGTGGTATGGTGGCAGTGCATAGGCCACTAGTGGATCTG |
| Delare2ev | AACAGACACATTACGTTAGCAAAAGCAACAATAACAAACACAACCCAGCTGAAGCTTCGTACGC |
| Are1fwctrl | GAAAAATGTGAGATGGTGTAGAGTG |
| Are1revctrl | ATGGTTCTGCCCCAGATTTACC |
| Are2fwctrl | CTTTCATCAATACATCTATATATTCG |
| Are2revctrl | GTAATTGTGGTAGCTGTGTGTTCAT |
| Primers for construction of overexpressing constructs | |
| Lpx1fw | GCGTGGATCCATGGAACAGAACAGGTTCAAGAAAG |
| Lpx1rev | CGGGAATTCTTACAGTTTTTGTTTAGTCGTTTTAACC |
| Ldh1fw | GCGTGGATCCATGAATATGGCAGAACGTGCAG |
| Ldh1rev | CGGGAATTCCTACAATTTGGAATTATCAATCACCTCTCG |
| Yju3fw | GCGTGGATCCATGGCTCCGTATCCATACAAAG |
| Yju3rev | CGGGAATTCTTATGGTTTAGCTTCGGTCGTG |
| Ayr1fw | GCGTGGATCCATGTCGGAGTTACAGTCACAAC |
| Ayr1rev | CGGGAATTCCTAATCGTCCTTATTCTTCTGTTTCGAC |
| Eht1fw | GCGTGGATCCATGTCAGAAGTTTCCAAATGGCC |
| Eht1rev | CGAGTCGACTCATACGACTAATTCATCAAACTTAGTG |
| Tsc10fw | GCGTGGATCCATGAAGTTTACGTTAGAAGACCAAGTTG |
| Tsc10rev | CGAGTCGACTCAGTTGGCCTTCTTGCCGTC |
| YBR056wfw | GCGTGGATCCATGATTGGCTCACTTAGAAACAAATTTGAG |
| YBR056wrev | CGGGAATTCTTAATACTTATTAAACTCATCTAACCCACGTTG |
| YKL050cfw | GCGTGGATCCATGTCACTAATATCTGCGTTGCAAAC |
| YKL050crev | CGAGTCGACTTAGATAACCTCTTTGAAAAAACTTTCCTTAGGAG |
| Primers for site-directed mutagenesis | |
| Ayr1_S18Afw | GTTGTTACAGGCGCCGCCGGTGGTATTGGATATG |
| Ayr1_S18Arev | CATATCCAATACCACCGGCGGCGGCTGTAACAACG |