TABLE 7.
Triacylglycerol mobilization of overexpressing strains in the presence of cerulenin
Strains overexpressing putative new lipases as well as the TM were grown in the presence of cerulenin. Culture aliquots were taken between 0 and 7 h and analyzed for TG content as described under “Experimental Procedures.” TG content at the starting point was set as 100% and followed over time. Results shown are the average from at least three independent experiments. S.D. values were <10%.
| Strain | TG content after 7 h |
|---|---|
| % of starting point | |
| Δtgl3/4/5 (VC) | 80 |
| TM + LPX1+ | 40 |
| TM + LDH1+ | 70 |
| TM + YJU3+ | 75 |
| TM + AYR1+ | 53 |
| TM + EHT1+ | 70 |
| TM + TSC10+ | 80 |
| TM + YBR056w+ | 70 |
| TM + YKL050c+ | 70 |