FIGURE 1.

A, schematic model of mRv (PDB ID code 1IKU) showing the arrangement of color-coded EF hands, the location of Cys-39 in EF-hand 1 (purple), and the N-terminally attached myristoyl group (orange, space filling). B, sequence alignment of the four EF hand loop regions of recoverin. The consensus sequence for EF hand loops is shown with the Ca²⁺-coordinating residues denoted as X, Y, and Z (underline indicates the negative position), n represents any non-polar residue, and a dash is any residue. The conserved Ca²⁺-binding residues are marked in red boldface. The gray box in EF1 highlights the conserved CPXG motif.