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. 2013 Nov 4;288(50):36160–36167. doi: 10.1074/jbc.M113.524355

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — Binding assays for WT or C39A Rv to RGS. A, pulldown assay using a Ni-NTA matrix to immobilize RGS as described under “Experimental Procedures.” The RGS-Rv complex was eluted with 250 mm imidazole. Lanes are defined as L, load; FT, flow-through; W, last wash; E, imidazole elution. B, ITC isotherm showing the heat observed upon injecting aliquots of WT Rv (660 μm) into a solution of RGS (60 μm) at 30 °C. C, ITC isotherm showing the heat observed upon injecting aliquots of C39A (340 μm) into a solution of RGS (30 μm) at 30 °C.