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Binding constants from titration data fit to the concerted allosteric model (Equation 3)
Errors were calculated using the least squares method (R2 > 0.99).
| Protein | Myr | Ca2+ binding |
||
|---|---|---|---|---|
| K1 | K2 | L | ||
| μm | μm | μm | ||
| WT | − | 0.15 | 2.4 | 0.31 ± 0.14 |
| + | 0.20 | 6.9 | 490 ± 30 | |
| C39A | − | 0.15 | 2.4 | 11.1 ± 0.8 |
| + | 0.20 | 6.9 | 280 ± 10 | |
