Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Dec 1;27(23):2531–2536. doi: 10.1101/gad.229195.113

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Welcker et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 3.0 Unported), as described at http://creativecommons.org/licenses/by-nc/3.0/.

PMC Copyright notice

Figure 2. — Degron determinants of dimer dependence. (A) 293A cells were cotransfected with MYC-cyclin E (mutations and their CPD positions, as indicated) and Flag-Fbw7 and analyzed as in Figure 1. (B) Weakened degrons containing serine in the central positions render cyclin E nearly resistant to degradation by Fbw7. The assay is as in A. (C) Introducing proline into P+2 of the T62 degron (D64P) results in a strong degron that overcomes the need for the T380 degron (D64P/T380A). The assay is as in A. (D) Schematic of the interaction of a cyclin E degron peptide with the Fbw7 propeller. (E) Fbw7 propeller mutants reveal mutational buffering by Fbw7 dimerization. Cells were cotransfected with Flag-Fbw7 dimer and monomer (ΔD) constructs containing mutations of degron-contacting residues as indicated and tested for MYC-cyclin E turnover by Western blotting as above.