Figure 3. The flagellar apparatus is required for Pch polar localization and maintenance of low c-di-GMP.
(A) Representative images (10.8 × 10.8 microns) of Pch-mCherry localization in fliF and flhF deletions. Refer to the ‘Materials and methods’ section for a description of contrast settings for both image types. (B) Histograms of cellular c-di-GMP concentrations in fliF and flhF deletions. Error bars represent counting error. The mean percentage of ΔfliF cells with less than 200 nM c-di-GMP from three biological replicates is 0.1% (standard deviation of 0.1%). The mean percentage of ΔflhF cells with less than 200 nM c-di-GMP from three biological replicates is 26.6% (standard deviation of 5.2%). (C) Quantification of the mean percentage of cells exhibiting polar localization of Pch-mCherry in ΔfliF and ΔflhF backgrounds from four biological replicates. Error bars represent the standard deviation. Strains contain empty vector (pVC) or a complementing plasmid. Quantification was performed as described in the ‘Materials and methods’. (D) A representative histogram of Pch-mCherry focus distance to the nearest cell pole in wild type and flhF deletion strains. Error bars depict counting error.
Figure 3—figure supplement 1. The flagellar apparatus is required for Pch-mCherry polar localization.
Figure 3—figure supplement 2. Quantitation of Pch.
