Figure 4. CheA is required for the maintenance of low c-di-GMP and polar localization of Pch-mCherry.
(A) Quantification of Pch-mCherry polar localization in different che mutants. Strains contain empty vector (pVC) or a complementing plasmid. (B) Quantification of c-di-GMP in different che mutants. Data plotted in (A) and (B) are from three biological replicates. Error bars in (A) and (B) depict standard deviation. (C) Representative images (10.8 × 10.8 microns) of Pch-mCherry localization in a cheA deletion. (D) A histogram of cellular c-di-GMP levels in ΔcheA cells. Error bars depict the counting error.