Fig. 3.

Site specificity within proteins. Total spectral counts (TSCs; red lines) for specific ubiquitin-modified lysines are plotted against the linear peptide sequence for the indicated protein. The log2 SILAC ratios (heavy (H) = treated with 1 μm Bortezomib; light (L) = untreated) for the same ubiquitin-modified lysines are shown in the bottom half of the protein-specific plots. The disorder tendency (black line) as predicted by IUPred (71) and the predicted membrane topology are plotted along the protein sequence. All primary data used for this figure were extracted from the published diGLY-modification data set of Kim et al. (22).