The phenotype of epitope-tagged bzr1-1D and BZR1 transgenic plants.
A, Diagrammatic representation of the constructs used to transform wild-type Col plants. bzr1-1D or BZR1 was fused to 7Myc-6His (MH) under the control of the 2×35S promoter. Blue boxes indicate Gateway recombination sites. B and C, Three-week-old 35S::bzr1-1D-MH (B) and 35S::BZR1-MH (C) plants displayed a similar phenotype to that of bzr1-1D or pBZR1::bzr1-1D-CFP, and Col-0 or pBZR1::BZR1-CFP control plants respectively. D, Dark growth of the indicated seedlings on 0.5× MS medium containing 2 μm propiconazole (PPZ) for 6 days revealed that the 35S::BZR1-MH seedlings were sensitive to PPZ whereas the 35S::bzr1-1D-MH seedlings were insensitive to PPZ, similar to pBZR1::BZR1-CFP or Col-0, and pBZR1::bzr1-1D-CFP or bzr1-1D plants, respectively.