Fig. 2.
BiFC assays in A. thaliana protoplasts. A. thaliana protoplasts were transfected with pSPYCE/pSPYNE vectors, as described in Sheen (34), to corroborate the in vivo interaction of Cc and its potential targets in BiFC. Images were captured 24 h following transient transfection and after 6 h of treatment with 35 mm H2O2. Reconstruction of eYFP leads to the obtainment of green fluorescence signal emission, indicative of interaction between Cc and its partners. Protoplasts transfected with chromatin-remodeling complex element SWI3B, a protein unable to interact with Cc, were used as a negative control. The nucleus was stained in blue using DAPI dye. Scale bar is 10 μm.