Disease symptoms on two kinds of cotton cultivars and representative 2-DE maps of total differential expression proteins isolated from the resistant cotton cultivar.
A, Infection of cotton seedlings with V. dahliae. After seeding for 2 weeks, the seedlings of G. barbadense cv7124 (resistant) and G. hirsutum cv.YZ-1 (susceptible) were dip-infected with the liquid containing V. dahliae spores and treated with sterile distilled water as a Mock treatment. At least 20 plants were used for V. dahliae inoculation per experiment and the experiment was repeated for three times at the same condition. The Mock and infected roots of 7124 harvested at 1, 6, 12, 24, 48, and 72 hpi were used for protein and RNA extraction. Photos were taken 14 d post inoculation. B, Protein spots of down-regulation, C, up-regulation and (D) other regulatory patterns are indicated in the representative 2-DE maps. The differently expressed proteins were labeled in the images.