Fig. 7.

The JA signaling pathway is suppressed in GbSSI2-silenced cotton and activated in cotton after inoculation with V. dahliae. A, Detection of JA signaling pathway-related genes in TRV:00 and TRV:GbSSI2 plants by qPCR. Error bars represent the standard deviation for three independent experiments, and three technical replicates were analyzed; asterisks indicate statistically significant differences, as determined by the Student t test (**p < 0.01). B, Detection of JA levels in TRV:00 and TRV:GbSSI2 plants. Error bars represent the standard deviation of three biological replicates (n = 10); asterisks indicate statistically significant differences, as determined by the Student t test (**p < 0.01). C, RT-PCR analysis of JA signaling pathway-related genes in G. barbadense cv7124 on inoculation with V. dahliae. PCR was performed by 28 cycles of amplification for GbUB7 and 29 cycles for other genes.