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Subtiligase-based identification of peptides derived from caspase cleavage. A, Schematic diagram of subtiligase-based substrate identification method. B, Sequence of synthesized peptide glycolate ester and cleavage sites for subtiligase and TEV protease. C, Nomenclature for residues in the cleavage sites of endopeptidase substrates. For caspase substrates, the P1 position is Asp.
