Fig. 7.

Functional validation of S100A4 knockdown in vitro. A, OCTT2 cells were transfected with siS100A4 for 48 h, after which spheroids were formed for 72 h and embedded into type I collagen. Invasion was significantly repressed at 72 h time point compared with control, as quantified in the graph (right panel). Representative 20× images of three independent experiments. ImagePro was used to quantitate the area of invasion (mm²). B, OCTT2 cells transfected with siS100A4 (48 h) were over-laid on top of FEF3-GFP 3-D organotypic matrix. Reconstructs were grown for 2 weeks. 40× images of representative H&E slides. C, Reconstructs stained with involucrin showed increased differentiation in the siS100A4 samples. Representative image of three independent experiments. n = 3, *p < 0.05.