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. 2013 Sep 16;12(12):3834–3850. doi: 10.1074/mcp.M112.021881

Fig. 3.

Fig. 3.

The sensitivity of fluorescent staining formulations. Derived from Fig. 2. A, Lowest limit of detection (LLD) was taken as the minimal amount of protein that could be discriminated from background. B, Lowest Practical Sensitivity (LPS) was taken as the maximum amount of protein that was indistinguishable from background. C, Integrated Measure of Practical Sensitivity (IMPS) was taken as the inverse product of LLD × LPS. * Indicate significant differences from SR (stain i). † Indicate significant differences from best performing commercial CB formulation by each measure of sensitivity (formulations xiv, xii and xii, in panels A, B, and C, respectively). ‡ Indicate significant differences from the best performing published CB formulation in each experiment (formulations xiv, xii and xii, in panels A, B, and C, respectively). § Indicates no significant differences between the commercial and published CB formulations providing the lowest LLD and LPS (formulations ii and xii in both parts A and B). £ Indicates a significant difference between commercial and published CB formulations providing the highest IMPS. (One-way ANOVA, F = 11.8, 22.7, 24.9 for parts A, B and C, respectively, p < 0.001, Post-hoc Tukey analysis, p < 0.05, n = 3). Error bars indicate S.D.