Fig. 3.

Recombinant P. falciparum merozoite proteins recapitulate known biochemical activities. The interactions between recombinant MSP1-MSP7 (A), and P12-P41 (B) were detected in both bait-prey orientations by screening the P. falciparum merozoite library with the indicated prey proteins using the AVEXIS assay. Baits labeled with an asterisk were below threshold levels required for the assay. Bar charts show mean ± S.D.; n = 3. C, Recombinant biotinylated EBA175 (top panel) and EBA140 (bottom panel) immobilized on fluorescent streptavidin-coated beads bound to untreated erythrocytes (blue line). Binding was blocked by pre-treating the erythrocytes with neuraminidase (red line) or, for EBA175, pre-incubating erythrocytes with an anti-GYPA monoclonal antibody (dotted green line). Negative controls were Cd4d3 + 4-coated beads (black line).