Table I. Details of the P. falciparum merozoite surface protein library. Each protein within the library is grouped according to its predicted subcellular localization (Sub-cell. Locn). The region of the protein (typically the full-length ectodomain) that was selected for expression is identified by the N- and C-terminal residues and their positions. The levels at which each protein was expressed was determined by ELISA against a known purified, quantified standard. All transfections were performed transiently using an unoptimized noncommercial transfection reagent, which routinely achieves 40–60% transfection efficiency. Expression levels are given as a guide only given the significant batch-to-batch variability observed using this approach and grouped into “high” (between 5 μg/ml–50 μg/ml:), “medium” (0.5 μg/ml–5 μg/ml) and “low” (0.005 μg/ml–0.5 μg/ml). The expression of nine proteins (PF3D7_1431400, EBL1, PTRAMP, RAP1, RAP2, RAP3, CLAG3.2, RhopH2, and RON3) could not be detected (n.d.). † Pf34 is also GPI-linked Prod: this is a footnote place after detected (n.d).
| Sub-cell. Locn | Official nomenclature | Synonym/s | Accession number | Region expressed | Len. (aa) | Exp. level |
|---|---|---|---|---|---|---|
| Surface (GPI-Anchored) | MSP1 | PFI1475w | V20-S1701 | 1682 | medium | |
| MSP2 | PFB0300c | I20-N246 | 227 | high | ||
| MSP4 | PFB0310c | Y29-S253 | 225 | high | ||
| MSP5 | PFB0305c | N22-S251 | 230 | high | ||
| MSP10 | PFF0995c | H27-S503 | 477 | medium | ||
| P12 | Pf12 | PFF0615c | H26-S323 | 298 | medium | |
| P12p | PFF0620c | Y21-T349 | 329 | low | ||
| P38 | Pf38 | PFE0395c | Q22-S328 | 307 | medium | |
| Pf92 | PF13_0338 | A26-S770 | 745 | low | ||
| Pf113 | PF14_0201 | Y23-K942 | 920 | medium | ||
| PF3D7_1136200 | PF11_0373 | L19-G656 | 638 | medium | ||
| PF3D7_1431400 | PF14_0293 | N25-S968 | 944 | n.d. | ||
| Peripheral | MSP3 | MSP3.1, SPAM | PF10_0345 | K26-H354 | 328 | high |
| MSP6 | MSP3.2 | PF10_0346 | Y17-N371 | 355 | low | |
| H101 | MSP3.3 | PF10_0347 | Q23-N424 | 402 | low | |
| MSP11 | H103, MSP3.7 | PF10_0352 | K27-Y405 | 379 | medium | |
| MSP7 | PF13_0197 | T28-M351 | 324 | high | ||
| MSRP1 | PF13_0196 | Y22-T379 | 358 | medium | ||
| MSRP2 | MAL13P1.174 | K24-T280 | 257 | low | ||
| MSRP3 | PF13_0193 | Q24-S298 | 275 | medium | ||
| P41 | Pf41 | PFD0240c | K21-S378 | 358 | high | |
| MSP9 | p101, ABRA | PFL1385c | N24-S742 | 719 | low | |
| Micronemal | AMA1 | Pf83, RMA1 | PF11_0344 | Q25-T541 | 517 | high |
| EBA140 | BAEBL | MAL13P1.60 | I26-P1135 | 1110 | low | |
| EBA175 | MAL7P1.176 | A21-P1424 | 1404 | low | ||
| EBA181 | JESEBL | PFA0125c | I27-S1488 | 1462 | low | |
| EBL1 | PF13_0115 | K22-N2584 | 2563 | n.d. | ||
| ASP | PFD0295c | A20-S708 | 689 | low | ||
| MTRAP | PF10_0281 | I23-K432 | 410 | medium | ||
| PTRAMP | TRAMP, TSP-3 | PFL0870w | N25-S306 | 282 | n.d. | |
| GAMA | PSOP9 | PF08_0008 | L22-P710 | 689 | medium | |
| Rhoptry | RH1 | RBP1, NBP1 | PFD0110w | Q24-T666 | 643 | very low |
| RH2b | RBP2b | MAL13P1.176 | H25-S1028 | 1003 | very low | |
| RH4 | RBP4 | PFD1150c | I27-T1148 | 1122 | very low | |
| RH5 | PFD1145c | F25-Q526 | 502 | low | ||
| RAP1 | PF14_0102 | I23-D782 | 760 | n.d. | ||
| RAP2 | PFE0080c | D22-L398 | 387 | n.d. | ||
| RAP3 | PFE0075c | N23-K400 | 378 | n.d. | ||
| CLAG3.2 | RhopH1(3.2) | PFC0110w | K21-H1416 | 1396 | n.d. | |
| RhopH2 | PFI1445w | L20-S1378 | 1359 | n.d. | ||
| RhopH3 | PFI0265c | K25-L897 | 873 | low | ||
| SPATR | PFB0570w | E22-C250 | 229 | high | ||
| AARP | PFD1105w | K18-P191 | 174 | low | ||
| Pf34† | PV2 | PFD0955w | N25-S306 | 282 | high | |
| RON3 | PFL2505c | N22-N249 | 228 | n.d. | ||
| RON6 | PFB0680w | F16-T949 | 934 | low | ||
| RAMA | MAL7P1.208 | Y17-K838 | 821 | low | ||
| Other | TLP | TRAP2 | PFF0800w | E24-P1306 | 1283 | low |
| PTEX150 | Pf112 | PF14_0344 | A20-N993 | 974 | low | |
| ETRAMP10.2 | PfJ323 | PF10_0323 | R25-R52 | 28 | medium | |
| PF3D7_0606800 | PFF0335c | V23-K299 | 277 | high |