Figure 2.

Olig2 expression in human mixed and radial glia cell (RGC) cultures. (A) Time-line of the cell cultures protocol used. (B–I) Olig2 (red) expression in mixed cell cultures in NG2⁺ cells at 24 h (B) and in PDGFRα⁺ early OPCs at 14 DIV (C). Olig2 expression in mixed cell cultures at 14 DIV was observed in O4⁺ late OPCs (D), in MAP2⁺ neurons (E) and in vimentin⁺ RGCs (F). Similar double-labeling results were demonstrated in RGC cultures after 14 DIV differentiation (G–I). Arrows show double-stained cells. (J) The percentages of neuronal (MAP2⁺), RGC (vimentin⁺) and OPCs (PDGFRα⁺, O4⁺) cells from total Olig2⁺ cells in control mixed cell cultures (14 and 17 gw), and in 17 gw RGC culture after 14 DIV. Scale bar: 20 μm.