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. 2013 Nov 6;4:31–38.

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Published by Library Publishing Media. This is an open access article, published under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5). This license permits non-commercial use, distribution and reproduction of the article, provided the original work is appropriately acknowledged with correct citation details.

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Figure 3. — A. Fibrinogenolytic activity of Naja kaouthia venom. SDS-PAGE of bovine fibrinogen (reduced) after incubation with 10µg crude Naja kaouthia venom at various time intervals. B. Inhibition of fibrinogenolytic activity of Naja kaouthia by polyvalent antivenom. The venom:polyvalent antivenom (1:1, w/w) mixture was pre-incubated for 1hr at 37°C. This mixture was incubated with 300μl of fibrinogen (2mg/ml) for 24hr and aliquots were withdrawn at different time interval and fractionated in 12.5% (w/v) SDS-PAGE. Lane 1. Undigested fibrinogen (control). Lane 2. Fibrinogen incubated with only venom; Lane 3. After 0.5hr; Lane 4. 1hr; Lane 5. After 2hr; Lane 6. After 4hr; Lane 7. After 8hr; Lane 8. After 16hr; and Lane 9. After 24hr.