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Nicotine withdrawal signs in wild-type mice after microinjection of mecamylamine into the IPN and VTA. A, Schematic of injection sites. B, Withdrawal signs after microinjection of saline (Sal, black bars) and 1 μg of mecamylamine (Meca, white bars) into the IPN (N = 6, 7, respectively) or VTA (N = 10). *p < 0.05 Sal versus Meca for same site and same chronic treatment (Neuman–Keuls post hoc comparisons). Error bars represent SEM.
