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. Author manuscript; available in PMC: 2013 Dec 13.
Published in final edited form as: J Biol Chem. 2003 Jan 30;278(17):10.1074/jbc.M211242200. doi: 10.1074/jbc.M211242200

Fig. 3. Cleavage site determination using MALDI-TOF mass spectrometry.

Fig. 3

One nanomole of peptide was incubated in the presence or absence of purified ECE-2 (5 ng) in 0.2 M sodium acetate buffer, pH 5.5, for 100 min at 37 °C. The reaction was terminated by quick freezing, and the samples were subjected to MALDI-TOF mass spectrometry as described (16, 28).