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. Author manuscript; available in PMC: 2013 Dec 13.
Published in final edited form as: J Biol Chem. 2003 Jan 30;278(17):10.1074/jbc.M211242200. doi: 10.1074/jbc.M211242200

Table II.

Hydrolysis of bioactive peptides by ECE-2: determination of cleavage sites by MALDI-TOF mass spectometry

Peptide Sequence and site of cleavage Hydrolysis
1. Big-Endothelin-1 graphic file with name nihms534032t1.jpg Partial
2. Bradykinin graphic file with name nihms534032t2.jpg Partial
3. des-Arg1-Bradykinin PPGFSPFR None
4. Neurotensin graphic file with name nihms534032t3.jpg Partial
5. Angiotensin I graphic file with name nihms534032t4.jpg Partial
6. Angiotensin II DRVYIHPF None
7. Angiotensin III RVYIHPF None
8. Substance P graphic file with name nihms534032t5.jpg Partial
9. LH-RH pEHWSYGLRPG-amide None
10. Arg-Vasopressin CYFQNCPRG-amide None
11. J-peptide EEEVAVGEGPGPRGDDAETGPRED-amide None
12. ACTH SYSMEHFRWGKPVGKKRRPVKVYPNVAENESAEAFPLEF None
13. β-endorphin YGGFMTSEKSQTPLVTLFKNAIIKNVHKKGQ None
14. Peptide E graphic file with name nihms534032t6.jpg Partial
15. BAM 22P graphic file with name nihms534032t7.jpg Partial
16. BAM 18P graphic file with name nihms534032t8.jpg Complete
17. Dynorphin B graphic file with name nihms534032t9.jpg Complete
18. Dynorphin A YGGFLRRIRPKLKWDNQ None
19. Dyn A-8 YGGFLRRI None
20. Little PEN-LEN graphic file with name nihms534032t10.jpg Partial
21. Big LEN LENSSPQAPARRLLPP None
22. PEN-22 AVDQDLGPEVPPENVLGALLRV None
23. PEN-21 AVDQDLGPEVPPENVLGALLR None
24. Big SAAS ARPVKEPRSLSAASAPLAETSTPLRL None
25. Little SAAS SAASAPLAETSTPLRL None
26. CPA5 N-term STNSFSYSSYHTLC None
27. CPA5 C-term CQTIMKHTLNHPY None
28. CPD N-term ALIKKAETVC None
29. CPE C-term KMMSETLNF None
30. CPE mid portion YDPDRPPCRKNDD None
31–42. Modified proCPE YEPGAPAAGXR
X=G,A,S,P,T,L,D,Q,M,F,Y,W		 None

The arrows represent primary cleavages and arrowheads represent secondary cleavages.