Fig. 2.

Quantitative analysis of the expression of the CIITA-PI, CIITA-PIII, and CIITA-PIV mRNA isoforms in human tumor cell lines. (A) Characterization of the pattern of usage of the different CIITA promoters in SK MEL-23, Me10538, M14, and U87 cells. (B) Measurement of CIITA-PIII and CIITA-PIV by qRT-PCR in Me10538 and M14 treated for 15 min, 3, 16, 24 and 48 h with either no IFNα or 250U/ml of IFNα. The results are expressed in copy number as the mean ± SEM of three independent experiments. In the absence of IFNα, the copy number of all transcripts did not vary significantly over the time tested.