Table 1.
Primers for quantitative PCR used in the study
| Transcript ID | Primer 5′→ 3′ | Annealing temperature (°C) | Product size (bp) | |
|---|---|---|---|---|
| GAPDH | G-F | AACGGATTTGGTCGTATTGGGC | 60 | 216 |
| G-R | TCGCTCCTGGAAGATGGTGATG | |||
| HLA-DRA | DRA-F | GGACAAAGCCAACCTGGAAA | 60 | 120 |
| DRA-R | AGGACGTTGGGCTCTCTCAG | |||
| HLA-DQA1*01a | A1 | CGGTGGCCTGAGTTCAGCAA | 63 | 158 |
| A-R | GGAGACTTGGAAAACACTGTGACC | |||
| HLA-DQA1*03a | A3 | CTCTGTTCCGCAGATTTAGAAGA | 60 | 151 |
| A-R | GGAGACTTGGAAAACACTGTGACC | |||
| CIITA | C2TA-F | CCGACACAGACACCATCAAC | 58 | 222 |
| C2TA-R | CTTTTCTGCCCAACTTCTGC | |||
| CIITA-PIII | P3-F | CCTGGCTCCACGCCCTG | 55 | 230 |
| P-R | GAACTGGTCGCAGTTGATG | |||
| CIITA-PIV | P4-F | GAGCTGGCGGGAGGGAG | 55 | 244 |
| P-R | GAACTGGTCGCAGTTGATG | |||
| IRF1 | IRF1-F | CCTGATACCTTCTCTGATGGACTCA | 60 | 182 |
| IRF1-R | CTGTCCGGCACAACTTCCAC | |||
| IRF2 | IRF2-F | GTCTACCGAATGCTGCCCCT | 60 | 276 |
| IRF2-R | AATGTCTGGCGGATTGGTGA | |||
| SOCS1 | SOCS1-F | GCAGCTGCACGGCTCCT | 60 | 195 |
| SOCS1-R | GGAGACTGCATTGTCGGCTG | |||
| SOCS3 | SOCS3-F | GCGAAGGCTCCTTTGTGGAC | 60 | 250 |
| SOCS3-R | GGGAAACTTGCTGTGGGTGA | |||
| SMD3-R | GGCGAACTCACACAGCTCCA | |||
a
HLA-DQA1- allele specific primers and internal standards were used in our experiments to measure copy number of DQA1-specific cDNA. The quantity of DQA1 transcript accumulated in each sample is the results the sum of copy numbers obtained using allele-specific primers. HLA-DQA1*01-specific couple of primers has been used for samples derived from Me10538 (DQA1*01/*03), M14 (DQA1*01/*03), U87 (DQA1*01/*01). HLA-DQA1*03-specific couple of primers has been used for samples derived from Me10538 and M14.