Fig. 3.
Effect of EGCG on in vitro growth of T. brucei. (A and B) WT BF cells were diluted back to 1 × 106 cells/ml into fresh media containing 0.1–1 μM EGCG or DMSO as the solvent control and culture cell densities were monitored for 6 days. (A) Representative growth curve showing cumulative culture densities. (B) Mean culture doubling times of 3 independent experiments. Error bars show ±SEM. (C) WT BF and (D) WT PF cells were back-diluted into fresh media containing 5–50 μM EGCG or DMSO control. Culture cell densities were determined after 48 h. Values are expressed as a percentage of DMSO control. The mean of 4 independent experiments is shown. Error bars show ±SEM. The * indicates P < 0.01 for the difference between DMSO and EGCG-treated conditions (student’s t-test).