Table 1.

Primer and probe sequences used for PCR-RFLP and high resolution melting assay.

Technique	Gene/SNP ID	Forward primer 5’ > 3’	Reverse primer 5’ > 3’	Probe 5’ > 3’
PCR–RFLP	dhfr.1	ATG GAA CAA GTC TGC GAC GTT TTC	ATG ACA TGT ATC TTT GTC ATC ATT
	dhfr.2	ATG GAA CAA GTC TGC GAC GTT TTC	ATT GTT ACT AGT ATA TAC ATC GCT
	dhps.1	CCATTCCTCATGTGTATA CAACAC	CATCTG AAACATCCAATTGTGT GA
	dhps.2	TATGATTCTTTT TCAGAT GGAGGT	CATCTGAAACATCCAATTGTG TGA
HRM	N51/C59	ACATTTAGAGGTCTAGGAAATAAAGGAGT	ATATTTACATCTCTTATATTTCAATTTTTC ATATTT TGATTCATTCAC	AAATGTAATTCCCTAGATATGAAATATTTTTGTG CAG-block
	I164	ACAAAGTTGAAGATCTAATAGTTTTACTTGGG	CTGGAAAAAATACATCACATTCATATGTACTATTTATTCTA	AATGTTTTATTATAGGAGGTTCCG-block
	S108	CTGTGGATAATGTAAATGATATGCCTAATTCTA	GACAATATAACATTTATCCTATTGCTTAAAGGT	GGAAGAACAAGCTGGGAAAGCAT-block
	S436/A437	GAATGTTTGAAATGATAAATGAAGGTGCTA	CAGGAAACAGCTATGACGAAATAATTGTAATACAGG TACTACTAAATCTCT	ATCCTCTGGTCCTTTTGTTATACC-block
	K540	GTGTTGATAATGATTTAGTTGATATATTAAATGATATTAGTGC	GTTTATCCATTGTATGTGGATTTCCTCTT	TAATCCAGAAATTATAAAATTATTAAAAAAAAA AAAC-block
	A581	CTTGTATTAAATGGAATACCTCGTTATAGGA	AGTGGATACTCATCATATACATGTATATTTTGTAAG	TTGGATTAGGATTTGCGAAGAAACATGAT CA-block
	A613	CTCTTACAAAATATACATGTATATGATGAGTATCCACTT	CATGTAATTTTTGTTGTGTATTTATTACAACATTTTGA	AAGATTTATTGCCCATTGCATGA-block