Table 3.
New malarial drug candidates.
| Inferred Plasmodium SL genes (Description) |
Yeast homologs of Plasmodium | Heterozygous knockout drug (Description) |
Homozygous knockout drug (Description) |
|---|---|---|---|
|
PB000415.02.0 (ADR gene: PfDHFR-TS) |
YOR074C | 5-Fluorouridine (antineoplastic), floxuridine (antiviral) | x |
|
PB000779.01.0 (PB000415.02.0’s neighbor gene) |
YDR334W | Caspofungin (antifungal) | Mechlorethamine (alkylating) |
|
PFD0830w (ADR gene: PfDHFR-TS) |
YOR074C | Methotrexate (antineoplastic antimetabolite), 5-fluorouridine (antineoplastic), floxuridine (antiviral) |
x |
|
PF08_0048 (PFD0830w’s neighbor gene) |
YDR334W | Caspofungin (antifungal) | Mechlorethamine (alkylating) |
|
PF13_0019 (ADR gene: PfNHE1) |
YHR081W | Miconazole (antifungal), fluconazole (antifungal), fenpropimorph (antifungal) | x |
|
MAL13P1.170 (PF13_0019’s neighbor gene) |
YOL115W | 5-fluorouracil (antineoplastic antimetabolite), 5-fluorouridine (antineoplastic) | x |
|
PVX_089950 (ADR gene: PfDHFR-TS) |
YOR074C | Methotrexate (antineoplastic antimetabolite), 5-fluorouridine (antineoplastic), floxuridine (antiviral) |
x |
|
PVX_089365 (PVX_089950’s neighbor gene) |
YLR085C | Alverine citrate (psychoactive) | Mechlorethamine (alkylating), busulfan (alkylating) |
|
PVX_118100 (ADR gene: PfMDR2) |
YMR301C | Fluconazole (antifungal) | x |
|
PVX_099360 (PVX_118100’s neighbor gene) |
YJL101C | Itraconazole (antifungal), methotrexate (antineoplastic antimetabolite) | x |
In future antimalarial drug research, a couple of the best candidates must be selected from the list, and they should be experimentally demonstrated to have the desired effect on the parasite. Drug target candidates were finally selected from ADR genes and their SL partners containing inferred Plasmodium (P. falciparum, P. vivax, and P. berghei) SL genes. We analyzed the fitness data for the yeast homologs of the selected Plasmodium genes to find new drugs for clinical malaria treatment. The right column of this table shows which drugs obtained from the top three experiments can be searched in the Drug Bank database.