Table 2.
Resistance allele frequencies for the F167Y, E198A and F200Y SNPs in isotype 1 beta-tubulin from pooled larval samples of Kirby and Wallangra isolates of H. contortus.
| Isolate | SNP position | Sequence direction | % Resistant allele (mean ± SE)a |
|---|---|---|---|
| Kirby | 167 | For | 0 |
| Rev | 4 ± 4 | ||
| 198 | For | 0 | |
| Rev | 0 | ||
| 200 | For | 7 + 3 | |
| Rev | 16 ± 3 | ||
| Wallangra | 167 | For | 0 |
| Rev | 2 ± 1 | ||
| 198 | For | 40 ± 2b | |
| Rev | 58 ± 2 | ||
| 200 | For | 32 ± 2b | |
| Rev | 38 ± 1 | ||
a
n = 3 separate pooled samples of larvae.
b
Denotes that within an isolate, and within a SNP position, the forward and reverse sequencing data were significantly different (P < 0.05) (data were not analysed where means of one or both sequencing directions were zero).