Figure 3. Changes in lipid composition of BFA-treated Chlamydomonas reinhardtii CC-503.

Cells were grown to the mid-log phase, treated with BFA (75 µg mL⁻¹) or DMSO (solvent control, Con) for 8 h, and then lipids were analyzed. (A) Accumulation of TAGs on a TLC plate as revealed by staining with primuline. The bands in the box are TAGs with the same Rf value as soybean TAGs. (B) BFA-treated cells accumulate higher amounts of TAGs than control cells. (C) Comparison of fatty acid compositions of TAGs isolated from BFA-treated and control (Con) cells. In (B) and (C), three replicates were averaged, and the SEs are shown. Significant differences, as determined by Student’s t-test, are indicated by asterisks (*P<0.05, **P<0.01). (D) Comparison of the major lipid classes between BFA-treated and control (Con) cells. Averages from two replicate experiments and their standard deviations are shown. PI, phosphatidylinositol; PE, phosphatidylethanolamine; PG, phosphoglyceride; DGTS, diacylglyceroltrimethylhomoserine; SQDG, sulfoquinovosyl-diacylglycerol; MGDG, monogalactosyldiacylglycerol; and DGDG, digalactosyldiacylglycerol.