Table 1. Primer and probe sequences for gene expression analysis of neuromarkers in the zebrafish brain.
Gene | forward 5' to 3' | reverse 5' to 3' | probe 5' to 3' |
---|---|---|---|
c-fos | GTATTACCCGCTCAACCAGAC | TCCAGTAACCCTCATTTTGGG | CGCAGCTCAATCCTACAACCCGA |
bdnf | AGCTGAAGAGACAACTTGCAG | CCATAGTAACGAACAGGATGGTC | TGCGCGGAGGTCTTATCCAAAACA |
gabra1 | ACTCTGCTTTACACCATGAGG | CTCGAGTCCAAACGTACACC | AAATTTGAGAGGGCAAGCATGGGC |
il10 | AGCTCCGTTCTGCATACAAAG | GGTCTCCAAGTAGAAATGCAGG | CAGTCCCTATGGATGTCACGTCATGAAC |
tub | ACTACACTATTGGCAAGGAGC | GAAACCCTGGAGACCTGTG | TGGTCAGACAGTTTGCGAACCCT |
c-fos: proto-oncogene c-fos, gabra1: gamma-aminobutyric acid receptor-A, bdnf: brain-derived neurotrophic factor, il10: interleukin-10