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Melt curves for unknown samples. Each peak shows the melting temperature for a different coccidian species. Red: Sarcocystis (cervid), Blue: Neospora/Hammondia, Orange: Sarcocystis (avian), Black: Cystoisospora, Green: Eimeria sp., Pink: Eimeria sp. The horizontal axis indicates melting temperature (°C) and the vertical axis [−d(RFU)/dT] is related to the amount of DNA present.
