Table 1. Percent similarity between amino acid sequences of CYPs.
Arabidopsis thaliana | Selaginella moellendorffii | ||||||||||
CYP85A2(BR6OX2) | CYP85A1(BR6OX1) | CYP90B1(DWF4) | CYP90A1(CPD) | CYP90C1(ROT3) | CYP90D1 | CYP90E1 | CYP90E2 | CYP90E3 | CYP90F1 | ||
Arabidopsis thaliana | CYP85A2 (BR6OX2) | 100 | |||||||||
CYP85A1 (BR6OX1) | 83 | 100 | |||||||||
CYP90B1 (DWF4) | 33 | 32 | 100 | ||||||||
CYP90A1 (CPD) | 37 | 35 | 42 | 100 | |||||||
CYP90C1 (ROT3) | 33 | 33 | 37 | 42 | 100 | ||||||
CYP90D1 | 35 | 35 | 36 | 41 | 52 | 100 | |||||
Selaginella moellendorffii | CYP90E1 | 32 | 31 | 34 | 40 | 38 | 37 | 100 | |||
CYP90E2 | 34 | 32 | 33 | 38 | 39 | 37 | 86 | 100 | |||
CYP90E3 | 33 | 32 | 34 | 38 | 37 | 38 | 77 | 81 | 100 | ||
CYP90F1 | 37 | 36 | 38 | 43 | 39 | 35 | 35 | 37 | 37 | 100 |
www.ncbi.nlm.nih.gov/blast/bl2seq/wblast2.cgi). Percent identity between Arabidopsis CYP90s ranged from 32% (between AtCYP90B1 and AtCYP85A1) to 83% (between AtCYP85A1 and AtCYP85A2). A comparison of CYP sequences in Arabidopsis and Selaginella revealed identity values ranging from 31% (between SmCYP90E1 and AtCYP85A1) to 43% (between SmCYP90F1 and AtCYP90A1). SmCYP90E consists of three members that share 77∼86% identity, whereas SmCYP90F has one member, which has 35∼37% identity with the SmCYP90Es. Sequences of known BR biosynthetic enzymes from Arabidopsis were pair-wise compared with select sequences from Selaginella. Percent identity values were calculated using the 2 BLAST program (