Figure 1. Representative DNA sequencing of NBS1 mutations in HCC and ICC.

(A) Missense NBS1 mutation at codon 638 (TCT→CCT, Ser→Pro) was identified in a case of ICC, but not in the adjacent non-tumorous tissue, indicating that the mutation was somatic. (B) Missense NBS1 mutation at codon 603 (TTC→TTA, Phe→Leu) in a case of HCC. The reverse complementary sequence is shown. (C) Synonymous NBS1 mutation at codon 90 (ACT→ACG, Thr→Thr) in a case of HBV-associated cirrhosis.