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. 2013 Dec 13;8(12):e83307. doi: 10.1371/journal.pone.0083307

Figure 1. HCV infection suppresses the expression of hepcidin, TfR1 and DMT1 mRNAs.

Figure 1

Naïve Huh7.5.1 cells were inoculated with media containing HCV particles or control media for 1–4 days post infection (d.p.i.). (A) The expression of HCV RNA was quantified by qPCR. (B) The expression of viral NS3 and core proteins and of cellular β-actin was analyzed by Western blotting. (C) The growth rate of uninfected control and HCV-infected cells was monitored by counting viable cells with the trypan blue exclusion method (n = 4 experiments). (D–E) The levels of hepcidin, TfR1 and DMT1(+IRE) mRNAs were analyzed by qPCR. After normalization with RPS18 values, mRNAs in HCV-infected cells were expressed relative to control cells at day 1. The graphs represented three independent experiments (means ±SD).